QuantiNova Multiplex RT-PCR Kit (2500)

Designed by QIAGEN for one-step real-time reverse transcription PCR, this master mix kit enables sensitive quantification of RNA targets using SYBR Green I or sequence-specific probes. A unique two-phase hot start system combined with a ready-to-use master mix buffer allows reaction setup at room temperature and supports highly sensitive qRT-PCR on any real-time cycler, while integrated safety features help eliminate variables and prevent artifacts during gene expression profiling.

The kit is intended for multiplex gene expression analysis of RNA targets and is compatible with real-time cyclers including the Rotor-Gene Q as well as instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche, and Agilent. To benefit fully from multiplexing, QIAGEN recommends an instrument that provides up to 5-plex capability, such as the Rotor-Gene Q.

An optional QuantiNova Internal Control RNA can be used to verify successful reverse transcription and amplification and to flag instrument or chemistry failures, assay setup errors, or the presence of inhibitors, with a visual pipetting control available to monitor correct pipetting. An integrated gDNA removal step prevents over-quantification of transcripts caused by genomic DNA carryover. The product is supplied for use in molecular biology workflows, is manufactured in Germany, and is stored at room temperature.