qPCR Services

QIAGEN's quantitative real-time PCR service delivers end-to-end gene expression profiling with rigorous quality control at each workflow stage. Submitted RNA samples undergo reverse transcription, primer validation, and amplification on validated instruments, using SYBR Green or probe-based detection chemistry tailored to each project. Typical turnaround spans 2 to 4 weeks depending on plate count and analysis depth.

Each engagement includes custom assay design, target optimization, and pathway-focused profiling through the RT² Profiler catalog, covering signaling cascades, disease panels, and receptor arrays. Bioinformatics deliverables feature raw Ct values, fold-change tables, and heatmap visualizations generated from the ΔΔCt method.

Researchers submitting cell lysates, tissue homogenates, or purified total RNA receive guidance on minimum input amounts, recommended storage at -80°C prior to shipment, and chain-of-custody documentation. The service supports applications including biomarker validation, compound screening follow-up, and pathway regulation studies across human, mouse, and rat samples. Results are returned as a comprehensive report along with raw amplification plots and a freezer-ready cDNA archive, formatted for direct upload into Ingenuity Pathway Analysis or comparable interpretation platforms.