QIAseq Targeted RNA Custom Panel (96) - 0 - 50 genes

A QIAGEN targeted RNA sequencing panel designed for custom gene expression profiling via an amplicon-based workflow. Published research applying the panel to measurable residual disease in acute myeloid leukemia describes a primer design in which each BC primer captures approximately 150-bp of target sequence and incorporates a UMI of 12 random nucleotides for molecular counting. The cDNA-based library preparation involves reverse transcription with gene-specific priming, target amplification, and final library amplification compatible with Illumina sequencing platforms, including the MiSeq and HiSeq 2500, using single-end 150 bp reads. The product belongs to QIAGEN's universal NGS consumables portfolio.