QIAseq Single Cell RNA Lib Kit UDID (96)

This RNA library preparation kit from QIAGEN is designed for RNA-seq library construction from single cells and low-input RNA samples for next-generation sequencing using Illumina NGS instruments. Built around a multiple-displacement amplification (MDA) workflow, the kit enables unbiased amplification and PCR-free NGS library construction, allowing reliable investigation of the transcriptome from single cells, low-input RNA samples, and small viral RNA genomes.

The procedure provides reagents to lyse cells, remove genomic DNA, perform reverse transcription using Quantiscript RT Enzyme Mix (60 minutes), ligate cDNA (30 min), isothermally amplify cDNA via MDA using a HiFi polymerase (120 minutes), enzymatically fragment the amplified cDNA, and generate a PCR-free library. Workflow steps are combined into a one-tube library preparation protocol that eliminates sample cleanup between steps, minimising starting-material loss and cross-contamination risk. The co-optimised MDA and library construction process is designed to be easily automated and to deliver high-diversity, NGS-ready libraries in one working day. The kit uses HiFi and proofreading polymerases, eliminating PCR amplifications and reducing PCR-induced duplication and PCR-based errors.

The system supports investigation of gene expression, transcript regulation, and small RNA genomes at single-cell resolution, and can also be used for specific enrichment of purified RNA. Amplified cDNA may be stored long-term at −30 to −15°C with no negative effects. The product is intended for molecular biology applications and is not intended for the diagnosis, prevention, or treatment of a disease. According to the manufacturer, the enzyme kit and UDI Y-Adapter kit should be stored at –30 to –15°C upon receipt, and QIAseq Beads should be stored at 2–8°C and never frozen.