QIAseq Single Cell RNA Lib Kit UDI (24)

QIAGEN's QIAseq Single Cell RNA Library Kit UDI is an end-to-end library preparation solution for RNA-seq from single cells, cell populations or small quantities of RNA. The kit includes all reagents required for cell lysis, reverse transcription, cDNA amplification and PCR-free NGS library preparation, including QIAseq Beads for reaction cleanup and QIAseq Unique Dual-Index Y-Adapter Kits for sample indexing. It produces high-quality NGS libraries and generates a tube of excess amplified cDNA that can be stored for follow-up experiments.

Highly suited for transcript discovery and differential gene expression from single eukaryotic cells, enriched cell populations and RNA-seq from limited samples, including viral RNA, the kit allows researchers to focus on mRNAs, explore the transcriptome (mRNAs and lncRNAs), or enrich for small RNA genomes such as viruses from low-input RNA samples. Multiple protocols are included with the kit, and RNA-seq data can be analyzed using the GeneGlobe-integrated RNA-seq Analysis Portal, a web-based data analysis solution included with this QIAseq Kit.

After cell lysis, genomic DNA degradation and reverse transcription, the cDNA is ligated to generate a long template amplified in a Multiple Displacement Amplification (MDA) reaction using REPLI-g SensiPhi DNA Polymerase, generating microgram amounts of long cDNA. This cDNA is randomly fragmented with QIAGEN's FX enzymatic fragmentation technology into short inserts suitable for sequencing. A single-tube library preparation then packages these inserts into NGS libraries which can be sequenced directly, without library amplification or additional PCR-bias. The complete cell-to-library workflow delivers highly complex PCR-free RNA-seq libraries from single cells in only 5.5 hours.