QIAseq FX DNA Library Kit HT A-D (384)

The QIAseq FX DNA Library Kit from QIAGEN provides unbiased enzymatic fragmentation for Illumina NGS workflows. According to the manufacturer, it achieves uniform coverage across GC-rich genomes in 2.5 hours using 10 pg–1 µg of input DNA. The kit is designed for whole-genome or metagenomic NGS library preparation, with or without PCR, and supports hybrid-capture target enrichment on Illumina instruments.

The kit includes buffers and reagents for DNA fragmentation (including end repair and A-addition), ligation, and library amplification. It provides plates containing unique dual index Y-adapters across Sets A, B, C, and D, along with reagents for library normalization. The manufacturer describes it as enabling all-enzymatic whole genome and hybrid capture library preparation for Illumina instruments with minimal bias.

The high-throughput configuration is engineered for consistent fragment size distribution, scalability, and automation compatibility with liquid handlers and plate-based workflows, suiting laboratories requiring reproducible NGS library generation.