QIAseq FastSelect RNA Lib Yeast Kit (24)

This probe-based ribosomal RNA depletion reagent from QIAGEN streamlines the removal of yeast rRNA during next-generation sequencing library construction, replacing traditional poly(A) selection when researchers need a more complete view of the transcriptome. The kit is tailored to Saccharomyces cerevisiae, targeting the 28S, 18S, 5.8S, and 5S rRNA species in a single, fast enzymatic step that integrates directly into existing RNA library prep workflows.

Validated for low-input samples and partially degraded material such as that recovered from formalin-fixed preparations, the workflow requires roughly 10 minutes of hands-on time and achieves >95% rRNA reduction when paired with magnetic-bead cleanup. The kit format supplies 24 reactions, suiting both individual yeast transcriptomics projects and small comparative studies where fungal gene-expression profiling is the goal. Resulting strand-specific libraries are compatible with Illumina sequencing platforms without buffer changes or additional purification steps.

Researchers can incorporate the reagent into QIAseq Stranded library preparation workflows, producing sequencing-ready libraries with reduced rRNA contamination and improved read mapping back to mRNA targets across yeast, hybrid, or host-microbe sample types.