QIAseq FastSelect RNA Lib Bac. Kit (96)

Bacterial ribosomal RNA depletion during next-generation sequencing library preparation is the central purpose of this 96-reaction kit from QIAGEN. The QIAseq FastSelect RNA Library Bacterial Kit uses a pool of probes that bind conserved rRNA sequences, allowing targeted enzymatic degradation in a single integrated step. This streamlined approach fits directly into standard RNA-seq library construction and can be completed in roughly 20 minutes of hands-on time per batch with minimal input RNA requirements.

Typical uses include bacterial transcriptome profiling, host-pathogen interaction studies, and shotgun metagenomic RNA sequencing. The kit is compatible with RNA extracted from both Gram-positive and Gram-negative bacteria, including diverse phyla, and integrates with common Illumina library prep chemistries. Reactions are performed in a single tube, reducing hands-on steps and the risk of sample mix-up.

Storage is at -20°C, and the provided reagents remain stable across multiple freeze-thaw cycles when handled properly. Each kit includes the FastSelect enzyme mix, blocking reagent, and internal control RNA, providing a complete workflow for bacterial rRNA depletion in research use only applications.