QIAseq DIRECT SARS-CoV-2 Kit F

A direct library-preparation kit from QIAGEN for SARS-CoV-2 sequencing, the QIAseq DIRECT SARS-CoV-2 Kit F offers a streamlined workflow to produce high-quality next-generation sequencing (NGS) libraries from viral RNA samples. It belongs to the QIAGEN UNGS_UNIVERSAL NGS portfolio within the Consumables budget line group, and is suitable for diagnostic research, genomic surveillance, and variant analysis.

The kit contains reagents for cDNA synthesis, SARS-CoV-2 target enrichment, and library amplification and indexing. The recommended RNA input is 5 µl, regardless of viral load in the sample extract. Amplification uses two primer pools producing overlapping amplicons of approximately 250 bp, so no fragmentation is required prior to sequencing on Illumina platforms. The kit provides 10 bp unique dual indexes (UDIs); with a paired-end read length of 149 cycles, sequencing time is just over 28 hours. Overall turnaround from viral RNA sample to sequencing is approximately five hours.