QIAseq DIRECT SARS-CoV-2 Kit E

A direct library-preparation kit from QIAGEN optimized for SARS-CoV-2 genomic sequencing. The kit streamlines conversion of viral RNA to sequencing-ready libraries for downstream NGS platforms and is designed for efficient workflow with low hands-on time and compatibility with standard Illumina sequencing. Recommended for surveillance, variant detection and research applications.

Per the associated enhanced protocol documentation, the workflow delivers an approximately five-hour turn-around time from viral RNA sample to sequencing and includes reagents for cDNA synthesis, SARS-CoV-2 enrichment, and library amplification and indexing. Amplification uses two primer pools producing overlapping amplicons of approximately 250 bp in size, and 10 bp unique dual indexes (UDIs) are included. RNA input is 5 µl, regardless of SC2 viral load in the sample extract. With a cycle read length of 149 for each paired-end read, the sequencing time is just over 28 hours.