QIAseq 16S/ITS Screening Panel (24)

Targeted next-generation sequencing of bacterial 16S rRNA and fungal ITS regions in a single workflow is enabled by QIAGEN's QIAseq 16S/ITS Screening Panel (24), an NGS consumable that supports simultaneous bacterial and fungal taxonomic profiling. The kit provides 24 indexed reactions with optimized primer pools, UMI-tagged adapters, and library amplification reagents compatible with Illumina sequencing platforms.

Key attributes include Unique Molecular Index tagging for accurate deduplication of PCR duplicates, primer designs targeting the 16S V3-V4 and ITS1/ITS2 variable regions, and a single-tube workflow that minimizes hands-on time and contamination risk. Input requirements start at approximately 1 ng of total microbial genomic DNA per reaction, suiting the assay for low-biomass sample types such as skin swabs, drinking water concentrates, and tissue-derived material.

Storage recommendation: -15 to -30 degrees C. Each kit contains individual reaction vials sufficient for 24 samples plus overage to support routine pipetting losses. The panel integrates with QIIME 2, mothur, or custom bioinformatic pipelines for genus-level taxonomic classification of paired-end Illumina reads.

This research use only kit is commonly applied across human gut microbiome studies, agricultural soil and rhizosphere characterization, fermented food monitoring, environmental water quality surveys, and clinical microbiome research where culture-independent profiling of both prokaryotic and eukaryotic microbial populations is needed within a single sequencing run.