QIAseq 12-Index L (48)

QIAGEN QIAseq Index Kits supply indexing adapters for use with QIAseq Targeted Panels, which enable Sample to Insight next-generation sequencing (NGS) of DNA or RNA and support ultrasensitive variant and clonotype detection from cells, tissue and biofluids using integrated Unique Molecular Indices (UMIs).

QIAGEN's Unique Dual Indexes (UDIs) are optimized for QIAseq Targeted Panel workflows, including QIAseq Targeted DNA, QIAseq RNAscan and QIAseq Immune Repertoire RNA Library Kits. Compatible only with Illumina instruments, these UDIs are designed to deliver high process safety and confidence in sequencing data by mitigating index hopping and mis-assignment of reads. The combination of UMIs and UDIs gives QIAseq Targeted Panels a remarkable level of accuracy. Combinatorial, non-unique dual index adapters are also available, and QIAseq Targeted DNA Indices can be used for both Illumina and Ion Torrent platforms.

The QIAseq Targeted DNA Panels use digital sequencing by incorporating molecular barcodes into starting DNA material before amplification, preserving the uniqueness of original DNA molecules and overcoming PCR duplicates, false positives and library bias. Isolated DNA, as low as 20 ng, is enzymatically fragmented, molecularly barcoded and enriched. The full workflow from extracted DNA to sequencing-ready libraries can be completed in 9 hours. Data can be analyzed with the QIAseq pipeline, a cloud-based solution that filters, maps and aligns reads, counts unique molecular barcodes associated with targeted genomic regions, and calls variants using a barcode-aware algorithm. These index kits are not suitable for QIAseq library prep kits that utilize Y-shaped adapter styles.