QIAquick Spin columns (100)

QIAGEN QIAquick spin columns are sold separately for use with the QIAquick PCR Purification, Gel Extraction, Nucleotide Removal and PCR & Gel Cleanup kits. They employ silica-membrane technology in a simple bind-wash-elute workflow, in which nucleic acids adsorb to the membrane under high-salt conditions, impurities are washed away, and pure DNA is eluted with a low-salt buffer or water.

The columns offer a binding capacity of 10 µg and can be used to purify DNA fragments ranging from 70 bp to 10 kb, with an elution volume of 30–50 µl and up to 80% recovery of DNA from gels or enzymatic reactions. They fit a conventional table-top microcentrifuge or a vacuum manifold with luer connectors, and the workflow can be automated on the QIAcube Connect.

Purified DNA is ready for direct use in downstream applications including sequencing, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.