QIAGEN OneStep RT-PCR Kit (1000)

Reverse transcription PCR workflows gain speed and consistency when cDNA synthesis and target amplification occur in a single closed tube. The QIAGEN OneStep RT-PCR Kit delivers 1000 reactions in this consolidated format, supplying the OneStep RT-PCR Enzyme Mix, 5x OneStep RT-PCR Buffer, dNTP Mix, and RNase-free water. The enzyme mix combines Omniscript and Sensiscript reverse transcriptases with HotStarTaq DNA Polymerase for hot-start activity that suppresses non-specific products.

Laboratories apply this kit to gene expression analysis, viral RNA detection, and validation of microarray data using total RNA or purified mRNA. Reactions typically use 20 uL volumes with 10 ng to 1 ug of total RNA input, and the chemistry supports amplicons up to 3 kb under standard cycling conditions. Store at -20 degrees C; avoid repeated freeze-thaw cycles to preserve reverse transcriptase and polymerase activity, and aliquot the enzyme mix for high-throughput labs.

By collapsing two enzymatic steps into one tube, this QIAGEN kit reduces pipetting errors, limits cross-contamination between cDNA synthesis and PCR setup, and shortens hands-on time for routine RT-PCR assays.