QIAGEN Multiplex PCR Plus Kit (100)

Engineered for straightforward and successful multiplex PCR in advanced applications, this kit from QIAGEN is built on proprietary multiplex PCR technology and delivers highly specific, sensitive amplification without the need to optimize PCR parameters. The preoptimized master mix combines HotStarTaq Plus DNA Polymerase with an innovative PCR buffer system developed specifically for multiplex PCR, helping to prevent amplification bias, primer-dimer formation, and nonspecific priming from the first attempt.

The kit supports DNA or cDNA starting material and a wide range of research, forensic, and molecular testing applications, including satellite DNA analysis (e.g., STR or VNTR analysis), typing of transgenic plants and animals, lineage analysis, GMO analysis, pathogen detection, food analysis, sex determination, mutation detection, amplification of SNP loci, qualitative and semiquantitative gene analysis, splicing isoform identification, hygiene analysis, microbial genotyping, metagenomic studies of environmental samples, pooling of singleplex assays, target enrichment for high-throughput sequencing of ancient DNA, and gene expression studies.

Four core components work together to streamline workflows. HotStarTaq Plus DNA Polymerase provides a stringent hot-start with a 5-minute activation at 95°C and enables room-temperature setup. Multiplex PCR Plus Buffer with Factor MP enables parallel amplification of all targets via a balanced KCl and (NH4)2SO4 combination, allowing a single protocol across multiplex assays. Q-Solution improves amplification of difficult templates such as GC-rich regions or those with complex secondary structure. CoralLoad Dye enhances pipetting visibility and allows direct gel loading with clear visualization of DNA migration. The ready-to-use master mix, which contains preoptimized concentrations of HotStarTaq Plus DNA Polymerase, MgCl2, and dNTPs, can be stored at 2–8°C, requires only primers and template to be added, and produces amplicons that can be analysed downstream by agarose gels, capillary sequencers, or the QIAxcel Advanced System.