QIAcuity Probe PCR Kit (25mL)

QIAGEN's QIAcuity Probe PCR Kit is a digital PCR (dPCR) reagent designed for the quantification of DNA or cDNA targets using TaqMan probes. The protocol supports both singleplex and multiplex reactions, accommodating up to 5 targets, and is run on QIAGEN's QIAcuity instruments with QIAcuity Nanoplates.

The 4x concentrated Probe PCR Master Mix contains a fluorescent reference dye for reliable detection of proper partition filling. For the highest efficiency in dPCR using TaqMan probes, amplicons should ideally be 60–150 bp in length. The kit contains QuantiNova DNA Polymerase, activated by an initial incubation of 2 min at 95°C, followed by 40 cycles of denaturation for 15 s at 95°C and combined annealing/extension for 30 s at 60°C.

The kit should be stored immediately upon receipt at –30 to –15°C in a constant-temperature freezer and protected from light; the master mix can also be stored protected from light at 2–8°C. Under these conditions, the components are stable for 12 months without showing any reduction in performance.