QIAamp MinElute ccfDNA Mini Kit (50)

From QIAGEN, this kit is designed for the isolation and purification of circulating cell-free DNA (ccfDNA) from human plasma or serum samples. The workflow combines pre-concentration of ccfDNA using magnetic beads with a subsequent column-based purification step on QIAamp MinElute columns, yielding highly concentrated nucleic acids suitable for sensitive downstream analyses such as liquid biopsy, PCR, qPCR, digital PCR, and next-generation sequencing.

The protocol accommodates scalable sample input volumes from 1 to 4 ml of serum or plasma and is optimized for the recovery of fragmented, low-abundance DNA — including small fragments as short as 20 base pairs — which enhances the detection sensitivity of low-frequency biomarkers. Purified DNA can be eluted in volumes as low as 20 µl, and the process efficiently removes proteins, salts, nucleases, cellular debris, and other inhibitors to generate pure nucleic acids compatible with demanding molecular applications.

For laboratories seeking higher throughput, the protocol can be partially automated on the QIAcube Connect for standardized processing of multiple samples. The kit is one component of the broader QIAGEN ccfDNA/RNA isolation portfolio, which addresses a range of sample types, volumes, and laboratory workflows.