QIAamp Fast DNA Tissue Kit, 50

QIAGEN's QIAamp Fast DNA Tissue Kit is designed for the rapid purification of genomic DNA from tissue samples, delivering results in approximately 30 minutes. Based on proven QIAGEN technologies, the kit overcomes common bottlenecks such as lengthy lysis protocols for fibrous or hard-to-lyse tissues by providing innovative Tissue Disruption Tubes containing a specially shaped bead that effectively disrupts tissue when agitated. Homogenization can be achieved in as little as 5 minutes on a desktop vortexer or 30 seconds in a high-powered bead mill.

The kit combines mechanical, chemical, and enzymatic lysis. The optimized chemistry allows homogenization and simultaneous stabilization of DNA released from the disrupted tissue. The digestion buffer mix also contains proteinase K, which is not affected by mechanical homogenization and completely lyses the sample material in a short subsequent incubation. Genomic DNA is subsequently purified using proven QIAamp technology, delivering pure DNA suitable for downstream applications such as PCR or next-generation sequencing. The procedure can be automated on the QIAcube or QIAcube Connect.

In addition to tissue samples, the kit can also be used for cells and blood using special protocols, which can be requested from QIAGEN Technical Service. Please note that bacteria cannot be used with this kit.