QIAamp DNA Mini Kit (250)

A DNA purification kit from QIAGEN (manufacturer Qiagen Gmbh) that provides a fast and easy method for isolating DNA from lymphocytes for reliable PCR and Southern blotting. The spin-column procedure uses a special lysate buffering condition that promotes optimal binding of DNA to the QIAamp membrane upon centrifugation. Salt and pH conditions in the lysate ensure that protein and other contaminants, which can inhibit PCR, are not retained on the QIAamp membrane. The bound DNA is washed in two centrifugation steps to ensure complete removal of any residual contaminants without affecting DNA binding, and the purified DNA is eluted from the QIAamp spin column using a buffered solution.

Categorised within QIAGEN's Primary Sample Technologies consumables portfolio, the kit is suited to DNA isolation from white blood cell preparations. According to a published protocol, expected DNA yields from white blood cells are up to 50 μg from 200 μL of cells at a count of 3.0 × 10⁶ to 1.0 × 10⁷. Buffers AL and AW1 contain guanidine hydrochloride, which can form highly reactive compounds when combined with bleach, and the kit is stored at room temperature and used before the manufacturer's expiration date.