Propidium Iodide, 10mg

A red-fluorescent nuclear and chromosome dye supplied by STEMCELL Technologies, commonly used as a viability dye for the detection of non-viable cells in a population. It functions as a DNA-labeling dye suitable for cell viability assessment, and is illustrated in flow cytometry analysis of human peripheral blood mononuclear cells labeled with the dye.

The compound is also known as Propidium diiodide and PI, with CAS number 25535-16-4. It is a crystalline solid with a molecular weight of 668.4 and a molecular formula of C₂₇H₃₄N₄ · 2I. Reported solubility values are approximately 2 mg/mL in PBS (pH 7.2), 0.2 mg/mL in EtOH, 2.5 mg/mL in DMSO, and 3.3 mg/mL in DMF. The product is intended for use as a laboratory reagent.

According to the manufacturer's safety information, the substance is classified as causing skin irritation, serious eye irritation, and may cause respiratory irritation; appropriate handling precautions and personal protective equipment are recommended.