Optiprep, 250mL

A sterile, ready-to-use density gradient medium supplied by STEMCELL Technologies, this 250 mL bottle delivers 60% (w/v) iodixanol in water for cell and particle separation by centrifugation. Researchers rely on the formulation for the isolation of mononuclear cells, granulocytes, platelets, and erythrocytes from peripheral blood, the enrichment of tissue-resident stem and progenitor populations, and the purification of subcellular organelles, viruses, lipoproteins, and exosomes using reproducible buoyant-density layering. Because the working solution arrives at a defined 60% concentration, dilutions with buffered saline generate predictable continuous or discontinuous gradient steps, simplifying sample preparation in flow cytometry workflows, immunohistochemistry feed-in studies, and downstream in vitro culture of separated fractions. The reagent is stored at room temperature and protected from light, and single-use aliquots are recommended once opened to preserve osmolarity and sterility across long isolation runs.