Ms Recom TRAIL, 10 µg

STEMCELL Technologies offers a recombinant form of mouse TRAIL, a TNF-related apoptosis-inducing ligand. The protein recruits the FAS-associated death domain and activates caspases 8 and 10, ultimately leading to apoptosis. Biological activity is tested by the protein's ability to inhibit the proliferation of RPMI-8226 cells, with cell proliferation measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum, and in the cited example is less than 100 ng/mL.

For purity analysis, 2 μg of the recombinant TRAIL is resolved with SDS-PAGE under reducing (+) conditions and visualized by Coomassie Blue staining. The product is designed for use in research areas including culture and evaluation of human mammary epithelial cells in CFU assays, culture of human mammospheres and tumorspheres, and as a cell culture supplement.