Ms Recom TNF-alpha, 20 µg

STEMCELL Technologies offers a recombinant form of the murine cytokine for use as a laboratory reagent in research settings. The product is manufactured under a quality management system certified to ISO 13485 and is intended for research use only, not for human or animal diagnostic or therapeutic uses unless otherwise stated.

The cytokine facilitates adhesion of neutrophils, monocytes, and memory T cells to activated platelets and endothelial cells. The biological activity of the recombinant protein is assessed by its ability to induce cytolysis of L929 cells growing in the presence of actinomycin D, with cell viability measured after 19 hours of culture using a fluorometric assay method. The EC50 is defined in this assay as the effective concentration of the growth factor at which cell viability is at 50% of maximum.

The material is supplied as a solid powder. According to its safety data sheet, it is not classified as a hazardous substance or mixture according to the Globally Harmonized System (GHS). When resolved by SDS-PAGE under reducing and non-reducing conditions and visualized by Coomassie Blue staining, the protein shows a predicted molecular mass of 17.4 kDa. Containers should be kept tightly closed in a dry, cool, and well-ventilated place.