Ms Recom Shh, 50 µg

Sonic hedgehog (Shh) is a member of the Hedgehog family of secreted signaling proteins with a prominent role in patterning during early mammalian development. It is expressed during early embryogenesis in a variety of tissues. During patterning of the central nervous system, Shh directly acts on cells to specify neural cell fates, regulates proliferation and survival of oligodendrocyte precursors and neural crest cells, and stimulates proliferation, differentiation, and axon growth. Shh also regulates T cell differentiation and activation.

Recombinant Mouse Shh from STEMCELL Technologies is a fully biologically active molecule expressed in E. coli with a predicted molecular mass of 19.8 kDa (Accession Q62226). The specific activity is ≥ 1 x 10^3 units/mg (EC50 ≤ 1 μg/mL), as determined by inducing alkaline phosphatase production of C3H/10T1/2 cells. In a separate assay using CCL-226 cells, the EC50 was 0.8 μg/mL. The product is ≥ 95% pure, with an endotoxin level ≤ 0.2 EU/μg protein as measured by kinetic limulus amebocyte lysate (LAL) analysis. It is supplied lyophilized after dialysis against phosphate-buffered saline.

For reconstitution, the vial should be centrifuged before opening and the product resuspended in sterile water to at least 0.1 mg/mL by pipetting the solution down the sides of the vial (do not vortex). Store at -80°C; the product is stable as supplied for 12 months from the date of receipt. Once reconstituted, store at 2–8°C for up to 2 weeks or at -20°C to -80°C for up to 3 months, avoiding repeated freeze-thaw cycles.