Ms Recom LIF, 50 µg

STEMCELL Technologies Mouse Recombinant LIF (Leukemia inhibitory factor) is an interleukin 6 class cytokine produced in E. coli with a predicted molecular mass of 19.9 kDa (UniProt accession P09056). The cytokine signals through the LIF receptor (LIFR), which associates with gp130 to activate JAK/STAT and MAPK pathways; LIFR-driven STAT3 activation is essential for maintaining the mouse embryonic stem cell phenotype. LIF also plays autocrine and paracrine roles in implantation by regulating proliferation, invasion, and differentiation of trophoblasts following blastocyst attachment, and is produced by CD4+ and activated regulatory T cells, where it promotes Foxp3 expression while repressing Th17 lineage-specific genes. Mouse LIF does not bind the human LIF receptor. Alternative designations listed by the manufacturer include CDF, D Factor, DIA, Differentiation-inducing factor, Differentiation inhibitory activity, Differentiation-stimulating factor, Emfilermin, Hepatocyte-stimulating factor III, HILDA, and MLPLI. LIF is also secreted by mesenchymal stromal cells, where it supports hematopoiesis and immune modulation. Cross-reactivity was not determined.

The product is supplied as a lyophilized powder, dialyzed against Tris buffer containing sodium chloride at pH 8.0, at a purity of ≥ 95%. Endotoxin level is ≤ 0.2 EU/μg protein as measured by kinetic Limulus amebocyte lysate (LAL) analysis. Specific activity is ≥ 1 x 10^8 units/mg (EC50 ≤ 0.01 ng/mL) as determined by a cell differentiation assay using M1 cells, with a representative EC50 of 0.0027 ng/mL (equivalently reported as 2.7 pg/mL). The lyophilized product is stable at -80°C for 12 months from the date of receipt and should be reconstituted in sterile water to at least 0.1 mg/mL by pipetting the solution down the sides of the vial without vortexing.

According to the manufacturer's safety data sheet, the material is classified as a non-hazardous solid powder and is intended for laboratory research use only. STEMCELL Technologies' quality management system is certified to ISO 13485.