Ms Recom IL-15, 10 µg

From STEMCELL Technologies, this Mouse Recombinant IL-15 (Interleukin-15) is a four alpha-helix bundle cytokine with many similar properties to IL-2, with which it shares components of its receptor. IL-15 is produced by antigen-presenting cells, T cells, and B cells, as well as epithelial and stromal cells. The IL-15 receptor is a heterotrimeric receptor composed of IL-15Ra (the high-affinity receptor for IL-15), as well as IL-2/IL-15Rb (CD122) and common gamma chain (CD132). IL-15 binds to IL-15Ra and can then be presented in trans to IL-2/IL-15Rb and common gamma chain on other cells; trans-presentation is thought to be the major mechanism by which IL-15-mediated responses occur in mice. The cytoplasmic domains of IL-2/IL-15Rb and common gamma chain mediate signaling to activate JAK/STAT and PI3K pathways. IL-15 supports the survival and proliferation of CD8+ T cells, promotes homeostasis of memory T cells, and promotes the survival and differentiation of NK cells as well as regulating their cytolytic activity.

Expressed in E. coli, the protein is lyophilized from a sterile-filtered aqueous solution containing sodium phosphate at pH 7.5. The specific activity is ≥ 5 x 10^4 units/mg, with an EC50 ≤ 20 ng/mL as determined by a cell proliferation assay using CTLL-2 cells. The product is ≥ 95% pure, with a predicted molecular mass of 13.4 kDa and UniProt accession number P48346. It shows cross-reactivity with human and rat samples. The endotoxin level is ≤ 1 EU/μg protein as measured by kinetic Limulus amebocyte lysate (LAL) analysis.

For preparation, centrifuge the vial before opening and reconstitute the product in sterile water to at least 0.1 mg/mL by pipetting the solution down the sides of the vial; do not vortex. Optionally, if the reconstituted product will not be used immediately, dilute with concentrated bovine serum albumin (BSA) to a final BSA concentration of 0.1%. Store lyophilized product at -20°C to -80°C; it is stable as supplied for 12 months from the date of receipt. For research use only.