Ms IgM, kappa Isotype Ctrl, MM-30, 50µg

STEMCELL Technologies supplies this mouse monoclonal isotype control antibody (Clone MM-30) of the IgM, kappa isotype, designed as an isotype-matched control for estimating the degree of non-specific binding by a primary antibody. An appropriate isotype control shares the same heavy chain subclass (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the antibody being tested; when a conjugated primary antibody is used, an isotype control conjugated to the same molecule (such as a fluorochrome) should be chosen. Use of a matched isotype control helps confirm the specificity of the antigen-specific antibody and reveals non-specific interactions such as binding to Fc receptors or other cell components.

The MM-30 antibody reportedly recognizes keyhole limpet hemocyanin and has been verified for use as an isotype control antibody in flow cytometry and immunofluorescence microscopy applications, including surface and intracellular staining. Supporting data include flow cytometry analysis of human H1 embryonic stem (ES) cells labelled with the unconjugated, Alexa Fluor® 488-conjugated, and PE-conjugated antibody, as well as immunofluorescence on H1 ES cells cultured in mTeSR™1 on Corning® Matrigel®-coated substrates.

The antibody is available in unconjugated, Alexa Fluor® 488, and PE conjugate formats.