Ms IgG2a,k Ctrl, MOPC-173, UC, 500µg

From STEMCELL Technologies, this unconjugated mouse monoclonal IgG2a, kappa isotype control antibody, Clone MOPC-173, is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen-specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being employed. If a conjugated antibody is employed, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody and indicates non-specific binding that may result from binding to Fc receptors or other cell components.

The MOPC-173 antibody is produced by a mineral oil-induced plasmacytoma cell line and has unknown binding specificity, having been screened on a variety of activated, resting, live and fixed tissues from several species, including mouse, rat, human, and non-human primates. The host species is mouse (BALB/c). This antibody clone has been verified for use as an isotype control for assessing non-specific binding to cells in flow cytometry and immunofluorescence microscopy applications, including surface and intracellular staining. Verified applications include FC, ICC, and IF, with reported applications including ChIP, FA, FC, ICC, IF, IHC, IP, and WB.

The product is formulated as a phosphate-buffered solution at pH 7.2 containing 0.09% sodium azide, and the antibody has been purified by affinity chromatography. The product is stable at 2–8°C when stored undiluted; do not freeze. The suggested use of this antibody is at concentrations comparable to those of the specific antibody of interest. Products are for research use only and are not intended for human or animal diagnostic or therapeutic uses unless otherwise stated.