Ms IgG1,k Ctrl, MOPC-21, UC, 500µg

STEMCELL Technologies offers this mouse IgG1, kappa isotype control antibody, Clone MOPC-21, which is suitable for use as an isotype-matched control in assays designed to estimate the degree of non-specific binding produced by an antigen-specific antibody. For an appropriate control, the isotype should share the same heavy-chain subclass (IgA, IgD, IgE, IgG, or IgM) and light-chain type (kappa or lambda) as the specific antibody in use, and when the primary reagent is conjugated, the matched isotype control should carry the same label (e.g., a fluorochrome). Including such a control helps confirm the specificity of the antigen-specific antibody and reveals non-specific interactions such as Fc-receptor binding.

The MOPC-21 clone has been verified as an isotype control for assessing non-specific binding to cells in flow cytometry and in immunofluorescence microscopy, including both surface and intracellular staining. The reagent is available unconjugated as well as conjugated to Alexa Fluor® 488, APC, FITC, and PE; manufacturer's supporting data additionally describe Biotin- and PerCP-Cy5.5-conjugated formats. Third-party product information further lists functional assays, immunocytochemistry, immunohistochemistry, immunoprecipitation, and Western blot among the applications for which this isotype control has been reported.