Mouse Immunoglobulin G2b

Purified from pooled normal mouse serum and supplied as a lyophilised powder, this unconjugated Mouse Immunoglobulin G2b (IgG2b) reagent from Antibodies.com serves as an isotype-matched standard and negative control in immunoassays where mouse IgG2b clones are the active binding reagent. The unconjugated format lets researchers conjugate the molecule directly to fluorophores, biotin, horseradish peroxidase, or alkaline phosphatase using standard amine- or thiol-coupling chemistry, supporting flow cytometry, western blot, immunohistochemistry, ELISA, and immunofluorescence protocols. Packaged in a 10 mg vial, the lyophilised form extends shelf life and simplifies shipping logistics; reconstituted material should be aliquoted and frozen to limit freeze-thaw cycles, with working solutions held at refrigerator temperatures for short-term use. Because the IgG2b subclass engages distinct Fc-receptor and complement pathways compared with IgG1, IgG2a, and IgG3, this preparation supports subclass-specific background subtraction, Fc-receptor binding studies, and matched-control experimental design. Researchers can apply it to block nonspecific Fc interactions in cell culture, to standardize plate-coating concentrations during immunoassay development, and to calibrate surface plasmon resonance or biolayer interferometry experiments involving mouse IgG2b capture antibodies.