Anti-IgE Antibody [1A2]

The Anti-IgE Antibody [1A2] from Antibodies.com is supplied as a liquid formulation at 100 µg, suitable for ELISA and Western blot applications in human immunological research. Clone 1A2 recognizes human immunoglobulin E, a key mediator of type I hypersensitivity reactions including allergic asthma, atopic dermatitis, and helminth infection responses. This unconjugated mouse IgG1 reagent enables flexible assay design, allowing researchers to pair it with secondary antibodies or detection chemistries of their choice. For optimal stability, store the vial at -20 °C in single-use aliquots and avoid repeated freeze-thaw cycles; brief refrigeration at 4 °C is acceptable for short-term working stocks. Recommended starting dilutions fall around 1 µg/mL for Western blot and 0.5-2 µg/mL for ELISA coating, though empirical optimization based on the target antigen is encouraged. The antibody specifically detects the human epsilon heavy chain, supporting studies of allergic disease mechanisms, mast cell activation, and therapeutic anti-IgE development such as omalizumab-related research.