miScript PreAMP Pathway Primer Mix

A preamplification primer mix from QIAGEN, part of the miScript miRNA PCR system, intended to support pathway-focused miRNA profiling when input RNA is limited. According to a phase-out notice issued by the manufacturer, production of miScript miRNA PCR products is scheduled to stop on May 1, 2021, with availability thereafter depending on remaining stock. A distributor catalogue groups the mix under the application category of RNA amplification from extremely small samples.

The broader miScript PreAMP range covers pathway, pathway-high-content, miRNome and custom primer mixes, together with dedicated PreAMP PCR Kits. The manufacturer recommends that users of miScript PCR migrate to the miRCURY LNA miRNA PCR systems, whose assays incorporate locked nucleic acid (LNA)-enhanced primers. The manufacturer states that these LNA-enhanced systems can quantify miRNA by qPCR with high sensitivity and specificity, and that the LNA design removes the need for an additional preamplification step in standard workflows.