miScript PreAMP Pathway HC Primer Mix

The HC (high-complexity) variant primer pool from QIAGEN is designed for multiplex pre-amplification of complex or expanded pathway target panels prior to miScript qPCR. According to the supplier, it is optimized for higher-complexity, multi-target panels, is intended to increase sensitivity and coverage while maintaining target representation, and reduces amplification bias to help preserve relative expression. The mix is described as integrating with QIAGEN miScript cDNA synthesis and qPCR assays, and is supplied for research use only; it is not for use in diagnostic procedures.

This primer mix is part of the broader miScript PreAMP product family from QIAGEN, which also includes Pathway, miRNome, and Custom Primer Mixes, alongside dedicated preamplification PCR Kits. QIAGEN has stated that miScript miRNA PCR products, including this HC Primer Mix, are being phased out, with production scheduled to stop on May 1, 2021, while stocks last; miScript PCR users are recommended to transition to the miRCURY LNA miRNA PCR systems. The manufacturer also notes that quantification of low-abundance miRNAs by qPCR usually requires a preamplification step such as that provided by this product.