miScript PreAMP miRNome Primer Mix

QIAGEN offers this preamplification primer mix as part of the miScript miRNA PCR system, used to amplify miRNAs from samples with low RNA content prior to qPCR profiling. The mix belongs to the PreAMP primer mix family, which addresses the challenge that miRNAs are frequently present at low abundance in common sample types, often necessitating a preamplification step.

QIAGEN discontinued production of the miScript miRNA PCR product line on May 1, 2021, with availability continuing only while stocks last. The recommended successor system is miRCURY LNA miRNA PCR, which incorporates locked nucleic acid (LNA) bases in optimally positioned locations to enable detection of biologically relevant miRNAs with high specificity and sensitivity without requiring a preamplification step. The miRCURY LNA miRNA PCR system is available in both SYBR Green-based and probe-based versions, combining the speed and convenience of a universal reverse transcription reaction with the sensitivity and specificity of LNA-enhanced qPCR primers.

For users transitioning from miScript products, the miRCURY LNA miRNA miRNome PCR Panels offer a comparable miRNome profiling solution. QIAGEN notes that for specialized applications still requiring preamplification, customers should contact their experts for further support.