miRNeasy FFPE Kit (50)

This QIAGEN kit is optimized for purifying microRNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The procedure uses silica-membrane spin column technology combined with a specialized lysis buffer that releases RNA from tissue sections while minimizing further degradation, and includes an optimized DNase digestion step to remove genomic DNA contamination.

The kit isolates RNA molecules from approximately 18 nucleotides upwards, providing recovery of usable RNA fragments, including miRNA and other small RNAs, for downstream applications such as PCR, qPCR, real-time RT-PCR, and microarray analysis. Specially optimized incubation conditions partially reverse formalin crosslinking of the released nucleic acids, improving RNA yield, quality, and performance in downstream enzymatic assays. Sample input can be up to four FFPE tissue sections, each with a thickness of up to 10 µm and a surface area of up to 250 mm² (the automated protocol on QIAcube supports up to two sections). Processing is performed manually, although a protocol for automated processing on QIAcube is available. Elution volume ranges from 14–30 µl of RNase-free water.

Kit components include RNeasy MinElute Spin Columns, collection tubes, Proteinase K, RNase-Free DNase I, DNase Booster Buffer, and a range of buffers for lysis, binding, and washing. Upon arrival, the RNase-Free DNase I and RNeasy MinElute spin columns should be stored at 2–8°C, while buffers can be kept at room temperature (15–25°C). The kit is intended for molecular biology applications and is not intended for the diagnosis, prevention, or treatment of a disease.