miRCURY LNA miRNA miRNome Probe Panels - Panel I - 4x Panel I; 4x 384-well plate

QIAGEN supports probe-based qPCR profiling of the human miRNome with the miRCURY LNA miRNA miRNome Probe Panels. Panel I contains 372 LNA Probe PCR assays for the amplification of human miRNAs, together with four inter-plate calibrators, three reference gene assays (U6snRNA, SNORD38B, and SNORD49A), and five RNA spike-in control assays that target the UniSp6 spike-in from the miRCURY LNA RT Kit and four additional RNA spike-ins (UniSp2, UniSp4, UniSp5, and cel-miR-39-3p).

The panels are supplied as dried-down LNA Probe PCR Assays in 96-well plate, 384-well plate, or Rotor-Disc 100 formats; the Panel I Rotor-Disc 100 configuration delivers the 372 assays across four Rotor-Discs with four blank wells on each disc. Locked nucleic acid (LNA) chemistry enables shorter primer and probe design with a Tm improvement of 2–8°C per base, supporting uniform detection of AU-rich miRNAs and improved mismatch discrimination for single-base specificity.

A universal reverse transcription reaction supports a single cDNA synthesis for any assay or panel setup, and the system workflow can be completed in just 2 hours from RNA to data. Compatible sample types include tissues, cells, exosomes, serum/plasma, urine, CSF, FFPE, buffy coat, PAXgene and cell line samples. The panels are intended for molecular biology applications.