miRCURY LNA miRNA Custom PCR Assay

QIAGEN's miRCURY LNA miRNA Custom PCR Assay provides custom-designed miRNA PCR primer sets that enable extremely sensitive and specific miRNA quantification. Both forward and reverse PCR amplification primers are miRNA-specific and optimized with LNA technology, providing higher binding affinity than standard DNA primers through Tm-normalization and LNA enhancement. Compared with miRNA PCR systems that use stem-loop or standard DNA primers, the LNA-enhanced primers offer significantly increased sensitivity, especially for AT-rich miRNAs.

The assays are provided in tube format. The upstream cDNA synthesis step uses the miRCURY LNA RT Kit, while digital PCR amplification is performed on the QIAcuity instrument with the QIAcuity EG PCR Kit. Universal RT makes it possible to use one first-strand cDNA synthesis reaction as the template for multiple miRNA real-time PCR assays. The custom design tool evaluates approximately 3,000 primer pair designs based on more than 60 different criteria to find LNA-optimal primer sets for miRNAs and other small RNAs 14–27 nucleotides in length.

Five validated reference gene primer sets are available for dPCR, targeting endogenous, small non-coding RNAs for data normalization. The QIAcuity system integrates partitioning, thermocycling and imaging in a single fully automated instrument, and remote analysis via Suite Software reports target concentration in copies per microliter with built-in quality control options.