MinElute Gel Extraction Kit (50)

QIAGEN's gel extraction kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments of 70 bp – 4 kb from up to 400 mg gel slices. Optimized for gel extraction of up to 5 μg DNA fragments in low elution volumes, the spin columns allow elution in very small volumes (as little as 10 µL), delivering high yields of highly concentrated DNA. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column.

DNA fragments purified with the MinElute system are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription. The kit removes nucleotides, enzymes, salts, agarose, ethidium bromide, and other impurities from DNA samples. A gel loading dye (GelPilot Loading Dye, containing xylene cyanol, bromophenol blue, and orange G) is provided for convenient sample analysis.

Spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as the QIAvac 24 Plus with QIAvac Luer Adapters. The kit can be fully automated on the QIAcube Connect, and for optimal results it is recommended to use this product together with QIAvac 24 Plus. The MinElute spin columns should be stored at 2–8°C upon arrival.