Investigator 24plex QS Kit (100)

QIAGEN's Investigator 24plex QS Kit is designed for human identification, enabling multiplex amplification of 22 autosomal STR markers that include the CODIS core loci and ESS markers, plus SE33, DYS391 and Amelogenin, alongside an integrated Quality Sensor. The kit uses fast-cycling PCR technology to deliver reliable results in approximately 45–60 minutes, with a recommended DNA input of 0.5 ng under standard conditions. Internal validations demonstrated robust and balanced results with 0.2 to 2 ng DNA and reliable results with <0.1 ng DNA.

Each amplification reaction has a volume of 25 μl, and the kit uses the BT6 matrix with six fluorescence labels (6-FAM, BTG, BTY, BTR2, BTO, BTP). The assay is compatible with Applied Biosystems 3500/3500xL Genetic Analyzers and can be analyzed using Applied Biosystems or GeneMapper ID-X software. The kit has been validated according to ENFSI and SWGDAM guidelines and meets ISO 18385 requirements.

Two Quality Sensors (QS1 and QS2) are co-amplified as positive controls, providing additional information on sample quality and PCR success. The Amelogenin and DYS391 gender markers have been optimized for small fragment sizes, supporting robust gender typing even with degraded or inhibited samples. The kit is suited for generating DNA profiles from blood, buccal swabs and forensic stains, and its data is authorized for upload to CODIS.