Human/Mouse Recombinant NT-3 (100 µg)

Neurotrophin-3 (NT-3) is a neurotrophic factor and member of the nerve growth factor (NGF) family of proteins that also includes NGF, brain-derived neurotrophic factor (BDNF), and neurotrophin-4/5. Offered by STEMCELL Technologies as a Human/Mouse recombinant protein, NT-3 signals through its transducing receptor tyrosine kinase TrkC and is known to promote the survival, development, and differentiation of neurons, as well as to modulate transmitter release at several types of synapses in the peripheral and central nervous systems.

The product is predicted to be a non-covalently linked homodimer, with each dimer having a molecular mass of 13.8 kDa for a predicted total molecular weight of 27.5 kDa. It is produced in E. coli and supplied lyophilized from a sterile-filtered aqueous solution containing 0.1% trifluoroacetic acid. Purity is ≥ 95% and endotoxin level is ≤ 1 EU/μg protein as measured by kinetic Limulus amebocyte lysate (LAL) analysis. For lot #1000050644 or higher, specific activity is ≥ 5.0 x 10^4 units/mg (EC50 ≤ 20 ng/mL), determined by a cell proliferation assay using a neuroblastoma cell line stably expressing TrkB (BR6); a representative EC50 in this assay is 9.3 ng/mL. For all other lots, specific activity is ≥ 50 units/mg (EC50 ≤ 20 μg/mL) as determined by a cell proliferation assay using C6 cells.

STEMCELL Technologies recommends storing the product at -20°C to -80°C, where it is stable as supplied for 12 months from the date of receipt. The vial should be centrifuged before opening and the contents reconstituted in sterile water to at least 0.1 mg/mL by pipetting the solution down the sides of the vial without vortexing. As an optional step, the reconstituted product may be diluted with concentrated bovine serum albumin (BSA) to a final BSA concentration of 0.1%.