Hu Recom VEGF-165, ACF, 1000 µg

Vascular endothelial growth factor (VEGF-165) is a heparin-binding homodimeric glycoprotein involved in embryonic vasculogenesis and angiogenesis. This animal component-free human recombinant VEGF-165 from STEMCELL Technologies is expressed in E. coli and purified to ≥ 95% purity, with endotoxin levels of ≤ 1 EU/μg protein as measured by kinetic Limulus amebocyte lysate analysis. The protein exists as a homodimer of 19.3 kDa subunits with a predicted molecular mass of 38.6 kDa, and each polypeptide chain contains 165 amino acids (UniProt accession P15692-4). It binds FLT1 (VEGFR-1) and KDR (VEGFR-2), activating the Raf/MEK/ERK and PI3K/AKT signalling pathways, and shows cross-reactivity with mouse and rat.

Biological activity is assessed by a cell proliferation assay using human umbilical vein endothelial cells (HUVECs), with a specific activity of ≥ 1.0 x 10^5 units/mg and an EC50 ≤ 10 ng/mL; a representative assay yielded an EC50 of 3.35 ng/mL after 88 hours of culture using a fluorometric readout. Beyond angiogenesis, VEGF plays a role in neurogenesis both in vitro and in vivo and exerts neurotrophic effects on neurons of the central nervous system, promoting the growth and survival of dopaminergic neurons and astrocytes. It also supports vascular endothelial cell survival, monocyte chemotaxis, and colony formation by granulocyte-macrophage progenitor cells. Alternative splicing of VEGF mRNA yields pro-angiogenic VEGFxxx and anti-angiogenic VEGFxxxb isoforms.

The product is supplied lyophilised from a sterile-filtered aqueous solution containing 0.1% trifluoroacetic acid and should be reconstituted in sterile water to at least 0.1 mg/mL without vortexing. It is stable as supplied for 12 months from the date of receipt when stored at -20°C to -80°C. STEMCELL Technologies' quality management system is certified to ISO 13485, and the product is intended for research use only.