Hu Recom VEGF-165, 1000 µg

STEMCELL Technologies supplies Human Recombinant VEGF-165 as a laboratory reagent for research use only; it is not intended for human or animal diagnostic or therapeutic uses unless otherwise stated. The product is presented as a solid powder and is not classified as a hazardous substance or mixture according to the Globally Harmonized System (GHS). It is manufactured under the supplier's ISO 13485-certified quality management system, with the supplier identified as STEMCELL Technologies Canada Inc., based in Vancouver, British Columbia, Canada.

VEGF is described as playing an important role in neurogenesis both in vitro and in vivo. The biological activity of this Human Recombinant VEGF-165 was tested by its ability to promote the proliferation of HUVECs, with cell proliferation measured using a fluorometric assay method. In the example cited, the EC50 — defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum — is 1.7 ng/mL.

SDS-PAGE analysis showed 4 μg of the protein resolved under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. The predicted molecular mass of Human Recombinant VEGF-165 is 38.2 kDa by non-reducing SDS-PAGE. The chemical is stable under normal conditions, with no hazardous reactions expected under normal processing.