Hu Recom VEGF-121, ACF, 10 µg

A vascular endothelial growth factor isoform expressed as a recombinant human protein for cell biology and regenerative medicine research, supplied as 10 µg of lyophilized powder. VEGF-121 is the freely soluble, 121-amino-acid splice variant of VEGF-A, which lacks the heparin-binding domains encoded by exons 6 and 7 and is therefore secreted rather than tethered to the cell surface or extracellular matrix. The animal component-free (ACF) production process avoids exposure to serum, BSA, or other animal-derived raw materials during fermentation and purification, supporting xeno-free workflows in endothelial differentiation, vascular organoid assembly, and preclinical cell-therapy development.

VEGF-121 drives proliferation, migration, and tube formation through high-affinity binding to VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1), and is commonly supplemented into endothelial cell culture media and embryoid body differentiation protocols. Researchers using this 10 µg format typically reconstitute the vial contents in sterile PBS or culture medium with 0.1% BSA carrier, then dilute into working concentrations in the single-digit ng/mL range to induce capillary-like sprouting in Matrigel or collagen angiogenesis assays. Aliquots should be stored at -20 °C or colder to preserve receptor-binding activity, with freeze-thaw cycles limited to a single round and light-protected handling between uses, since the cytokine is conformation-sensitive.

The preparation is supplied in a single 10 µg vial intended for research use only in in vitro studies of angiogenesis, hematopoietic stem cell maintenance, and tissue-engineered vascular networks. STEMCELL Technologies manufactures this cytokine under defined, animal component-free conditions to deliver consistent bioactivity lot to lot, and the protein is validated for use across the company's endothelial and hematopoietic cell culture and differentiation reagents.