Hu Recom TNF-alpha, 50 µg

A laboratory reagent supplied by STEMCELL Technologies, this recombinant human cytokine is intended for research use only and is not intended for human or animal diagnostic or therapeutic uses unless otherwise stated. TNF-alpha is referenced in the manufacturer's documentation as facilitating adhesion of neutrophils, monocytes, and memory T cells to activated platelets and endothelial cells.

Biological activity of Human Recombinant TNF-alpha is evaluated by the ability to induce cytolysis of L929 cells growing in the presence of actinomycin D, with cell viability measured after 48 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration at which cell viability is at 50% of maximum. SDS-PAGE analysis resolves 1 μg of the protein under reducing and non-reducing conditions with Coomassie Blue staining, and the predicted molecular mass is 17.5 kDa.

The product is classified as not a hazardous substance or mixture according to the Globally Harmonized System (GHS). It is supplied as a solid powder. Recommended storage is to keep containers tightly closed in a dry, cool and well-ventilated place. Documented applications include in vitro inflammation and signaling assays, cytotoxicity testing, and immune activation studies.