Hu Recom TNF-alpha, 10 µg

STEMCELL Technologies supplies Human Recombinant TNF-alpha as a laboratory reagent in solid powder form. The product is not classified as a hazardous substance or mixture according to the Globally Harmonized System (GHS), and the manufacturer's quality management system is certified to ISO 13485. It is intended for research use only and is not for human or animal diagnostic or therapeutic applications unless otherwise stated.

TNF-alpha facilitates adhesion of neutrophils, monocytes, and memory T cells to activated platelets and endothelial cells. The biological activity of Human Recombinant TNF-alpha was tested by its ability to induce cytolysis of L929 cells growing in the presence of actinomycin D, with cell viability measured after 48 hours of culture using a fluorometric assay method. The EC50 in the above example is 0.4 0.6 ng/mL. The protein has a predicted molecular mass of 17.5 kDa, and 1 μg was resolved with SDS-PAGE under reducing and non-reducing conditions and visualized by Coomassie Blue staining.

For safe handling, containers should be kept tightly closed in a dry, cool, and well-ventilated place. In the event of exposure, skin should be washed with soap and water, eyes rinsed thoroughly with plenty of water for at least 15 minutes, and fresh air sought following inhalation. The supplier, STEMCELL Technologies Canada Inc., can be reached at emergency telephone 1-800-667-0322.