Hu Recom TGF-beta 2, 50 µg

STEMCELL Technologies supplies Human Recombinant TGF-beta 2, a pleiotropic growth factor that is a member of the TGF-β superfamily. The protein signals through serine-threonine kinase type I and II receptors and activates signal transduction via SMAD family proteins, thereby regulating a variety of cellular functions including cell proliferation, differentiation, wound healing, apoptosis, and metabolism. TGF-beta 2 is also important in many developmental processes, as mice with TGF-β2 deletions show defects in the development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems.

The recombinant protein is produced from human cells and is supplied as a lyophilized preparation from a filtered solution containing hydrochloric acid. It has a predicted molecular mass of 12.7 kDa and an Accession Number of P61812. The product is also known by the alternative names BSC-1 cell growth inhibitor, Cetermin, Glioblastoma-derived T-cell suppressor factor, G-TSF, Polyergin, and TGFB2. Purity is reported as ≥ 95%.

Biological activity is determined by the ability of the protein to inhibit IL-4-dependent proliferation of TF-1 human erythroleukemic cells, yielding a specific activity of ≥ 5.5 x 10^6 units/mg with an EC50 ≤ 0.18 ng/mL. The endotoxin level is ≤ 1 EU/μg protein as measured by kinetic Limulus amebocyte lysate (LAL) analysis. The product should be stored at -80°C and is stable as supplied for 12 months from the date of receipt. For reconstitution, the vial should be centrifuged before opening and the contents reconstituted in sterile water to at least 0.1 mg/mL by pipetting the solution down the sides of the vial without vortexing. This product is for research use only.