Hu Recom RANKL, ACF, 10 µg

Supplied by STEMCELL Technologies, receptor activator of nuclear factor kappa-B ligand (RANKL) is a member of the tumor necrosis factor (TNF) superfamily. It is expressed as a type II homotrimeric transmembrane protein in both membrane-bound and secreted forms. RANKL binds to RANK and activates AKT signaling; osteoprotegerin (OPG) may also bind RANKL and compete with RANKL-RANK binding. RANKL is involved in osteoclastogenesis and T cell activation. The product is animal component-free.

This human recombinant protein is expressed in E. coli with a predicted molecular mass of 19.7 kDa and a purity of ≥ 95%. The specific activity is ≥ 2 x 10^4 units/mg, determined by ability to induce bioluminescence in engineered RANKL bioassay cells (EC50 ≤ 50 ng/mL). Endotoxin level is ≤ 1 EU/μg protein. The product is supplied lyophilized from a sterile-filtered solution containing sodium phosphate, pH 7.5. Store at -20 to -80°C; stable as supplied for 12 months from date of receipt. Centrifuge the vial before opening and reconstitute in sterile water to at least 0.1 mg/mL by pipetting down the sides of the vial; do not vortex. For research use only.