Hu Recom PDGF-BB, 1000 µg

A laboratory reagent from STEMCELL Technologies, Human Recombinant PDGF-BB is supplied as a solid and is intended for research use. According to the manufacturer's Safety Data Sheet, the product is not classified as hazardous under the 2012 OSHA Hazard Communication Standard (29 CFR 1910.1200) or the Globally Harmonized System (GHS), and is not regulated for transport by DOT, TDG, MEX, ICAO, IATA, IMDG, RID, ADR, or ADN. Storage conditions are described in the Product Information Sheet. The supplier is STEMCELL Technologies Canada Inc., based in Vancouver, British Columbia, Canada, and an emergency contact number is provided as 1-800-667-0322.

PDGF is described by the manufacturer as a mitogen for dermal fibroblasts, glial cells, arterial smooth muscle cells, epithelial cells, and endothelial cells. The biological activity of Human Recombinant PDGF-BB has been tested by its ability to promote the proliferation of NR6R-3T3 cells, with cell proliferation measured using a fluorometric assay. The EC50, defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum, was 2.12 ng/mL in the example provided by the manufacturer. The product is also referenced in the context of tyrosine kinase inhibitor research, with listed targets including KDR, PDGFR, KIT, RET, FLT-3, ABL, and ALK.

Under SDS-PAGE analysis, 1 μg of Human Recombinant PDGF-BB resolved under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining shows a homodimer of 12.4 kDa subunits with a predicted total molecular mass of 24.9 kDa.