Hu Recom PDGF-AB, ACF, 10 µg

A recombinant human platelet-derived growth factor AB isoform produced in an animal component-free (ACF) expression system, supplied as 10 µg of lyophilized protein in a single vial. PDGF-AB is a disulfide-linked heterodimer that signals through PDGF receptor alpha and beta, driving proliferation, migration, and chemotaxis in connective tissue cells, mesenchymal stem cells, and vascular smooth muscle cells in vitro. Researchers reconstitute the vial with sterile buffer such as PBS plus carrier protein and add it to serum-free or defined cell culture media at working concentrations typically ranging from 1 to 20 ng/mL depending on cell type and protocol. Common applications include expansion of mesenchymal stromal cells, maintenance of neural crest-derived lineages, fibroblast proliferation assays, and studies of wound healing, vascular remodeling, and fibrotic disease mechanisms. The ACF manufacturing process eliminates exposure to animal-derived raw materials during production, which simplifies downstream documentation for cell therapy and regenerative medicine workflows. For handling, store the lyophilized vial frozen at -20 °C and protect from light; after reconstitution, aliquot into single-use working volumes to limit freeze-thaw cycles. STEMCELL Technologies provides this cytokine as a research use only reagent, intended for laboratory investigation of growth factor signaling, stem cell self-renewal, and tissue engineering protocols.