Hu Recom Noggin, 1000 µg

Produced by STEMCELL Technologies, this recombinant human protein is expressed in CHO cells and functions as a bone morphogenetic protein (BMP) antagonist that binds BMP ligands of the transforming growth factor beta (TGF-β) family. It supports maintenance of undifferentiated human embryonic stem cells in vitro and can be used to prevent spontaneous differentiation in the short term. The protein is essential for development of structures derived from ectoderm, embryonic somite, skeletal patterning, and neurogenesis in vivo, and influences chondrogenesis, osteogenesis, and joint formation. It also promotes dopaminergic differentiation of embryonic stem cells and subsequent survival of dopamine neurons.

Alternative names include NOG, SYM1, and SYNS1, with accession number Q13253. The product is supplied lyophilized after dialysis against phosphate-buffered saline, with a predicted molecular mass of 30 kDa and a purity of ≥ 95%. The specific activity is ≥ 4 x 10^5 units/mg (EC50 ≤ 2.5 ng/mL), as determined by a bioassay using ATDC5 cells in the presence of 10 ng/mL human BMP-4. Endotoxin level is ≤ 0.2 EU/μg protein, measured by kinetic Limulus amebocyte lysate (LAL) analysis.

Store at -80°C; the product is stable as supplied for 12 months from date of receipt. To prepare, centrifuge the vial before opening and reconstitute in sterile water or phosphate-buffered saline to at least 0.1 mg/mL by pipetting the solution down the sides of the vial. Do not vortex.